Ausgewählte Publikationen

GM crops and organic farming
Ryffel,G.U. (2010). Making the most of GM potatoes. Nat. Biotechnol. 28, 318.

Ryffel,G.U. (2011). Dismay with GM maize. A science-based solution to public resistance against genetically modified crops that could be compatible with organic farming. EMBO Rep. 12: 996-999.

Ryffel,G.U. (2012). Orgenic plants: Gene-manipulated plants compatible with organic farming. Biotechnol. J. 11: 1328-1331.

Egg aging, a possible problem in artificial reproduction techniques
Kosubek, A., Klein-Hitpass, L., Rademacher, K., Horsthemke, B. and Ryffel, G.U. (2010). Aging of Xenopus tropicalis eggs leads to deadenylation of a specific set of maternal mRNAs and loss of developmental potential. PLoS ONE, 5, e13532.

Transgenic Xenopus as versatile tool to understand organogenesis
Ryffel, G.U., Lingott, A. (2000). Distinct promoter elements mediate endodermal and mesodermal expression of the HNF1a promoter in transgenic Xenopus. Mech. Dev., 90: 65-75.

Werdien, D., Peiler, G., Ryffel, G.U. (2001). FLP and Cre recombinase function in Xenopus embryos. Nucleic Acids Res. 29: e53.

Ryffel, G.U., Werdien, D., Turan, G., Gerhards, A., Gooßes, S., Senkel, S. (2003). Tagging muscle cell lineages in development and tail regeneration using Cre recombinase in transgenic Xenopus. Nucleic Acids Res. 31: e44.

Waldner, C., Sakamaki, K., Ueno, N., Turan, G., and Ryffel, G.U. (2006). Transgenic Xenopus laevis strain expressing Cre recombinase in muscle cells. Dev. Dyn. 235, 2220-2228.

Roose, M., Sauert, K., Turan, G., Solomentsew, N., Werdien, D., Pramanik, K., Senkel, S., Ryffel, G.U., and Waldner, C. (2009). Heat-shock inducible Cre strains to study organogenesis in transgenic Xenopus laevis. Transgenic Res. 18, 595-605.

Waldner, C.; Roose, M.; Ryffel, G.U. (2009). Red fluorescent Xenopus laevis: a new tool for grafting analysis. BMC. Dev. Biol. 9, 37.

Critical role of the transcription factor HNF1ß in nephrogenesis
Wild, W., Pogge v. Strandmann, E., Nastos, A., Senkel, S., Lingott-Frieg, A., Bulman, M., Bingham, C., Ellard, S., Hattersley, A.T., Ryffel, G.U. (2000). The mutated human gene encoding hepatocyte nuclear factor 1ß inhibits kidney formation in developing Xenopus embryos. Proc. Natl. Acad. Sci. USA, 97: 4695-4700.

Bohn, S., Thomas, H., Turan, G., Ellard, S., Bingham, C., Hattersley, A.T. and Ryffel, G.U. (2003). Distinct molecular and morphogenetic properties of mutations in the human HNF1ß gene that lead to defective kidney development. J. Am. Soc. Nephrol., 14: 2033-2041.

Dudziak, K., Mottalebi, N., Senkel, S., Edghill, E.L., Stefan Rosengarten, S., Roose, M., Bingham, C., Ellard, S. and Ryffel, G.U. (2008). Transcription factor HNF1ß and novel partners affect nephrogenesis. Kidney Int. 74, 210-217.

Drews, C., Senkel, S. and Ryffel, G.U. (2011). The nephrogenic potential of the transcription factors osr1, osr2, hnf1b, lhx1 and pax8 assessed in Xenopus animal caps. BMC.Dev Biol, 11, 5.

Sauert, K., Kahnert, S., Roose, M., Gull, M., Brändli, A.W., Ryffel, G.U. and Waldner, C. (2012). Heat-shock mediated overexpression of HNF1beta mutations has differential effects on gene expression in the Xenopus pronephric kidney. PLOS One, 7: e33522.

Dysfunction of the transcription factor HNF4a in diabetes and renal cell carcinoma
Drewes, T., Senkel, S., Holewa, B., Ryffel, G.U. (1996) Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes. Mol. Cell. Biol. 16:925-931.

Lausen, J., Thomas, H., Lemm, I., Bulman, M., Borgschulze, M., Lingott, A., Hattersley, A.T., Ryffel, G.U. (2000). Naturally occurring mutations in the human HNF4a gene impair the function of the transcription factor to a varying degree. Nucleic Acids Res. 28: 430-437.

Thomas, H., Jaschkowitz, K., Bulman, M., Frayling, T.M., Mitchell, S.M.S., Roosen, S., Lingott-Frieg, A., Tack, C.J., Ellard, S., Ryffel, G.U., Hattersley, A.T. (2001). A distant upstream promoter of the HNF-4a gene connects the transcription factors involved in maturity onset diabetes of the young. Hum. Mol. Genet. 10: 2089-2097.

Thomas, H., Senkel, S., Erdmann, S., Arndt, T., Turan, G., Klein-Hitpass, L. and Ryffel, G.U. (2004). Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4a and HNF1ß in a pancreatic ß-cell line. Nucl. Acids Res. 32, e150.

Lucas, B.; Grigo, K.; Erdmann, S.; Lausen, J.; Klein-Hitpass, L. and Ryffel, G.U. (2005). HNF4a reduces proliferation of kidney cells and affects genes deregulated in renal cell carcinoma. Oncogene, 24, 6418-6431.

Grigo, G., Wirsing, A., Lucas, B., Klein-Hitpass, L. and Ryffel, G.U. (2008). HNF4a orchestrates a set of 14 genes to down-regulate cell proliferation in kidney cells. Biol. Chem. 389,179-187.

Wirsing, A., Johnstone, K.A., Harries, L.W., Ellard, S., Ryffel, G.U., Stanik, J., Gasperikova, D., Klimes, I. and Murphy, R. (2010). Novel monogenic diabetes mutations in the P2 promoter of the HNF4A gene are associated with impaired function in vitro. Diabet. Med. 27: 631-635.

Waldner, C.; Rempel, O.; Schütte, F.; Yanik, M.; Solomentsew, N. and Ryffel, G.U. (2011). Double conditional human embryonic kidney cell line based on FLP and phiC31 mediated transgene integration. BMC Res. Notes 4: 420.

Wirsing,A., Senkel,S., Klein-Hitpass,L. and Ryffel,G.U. (2011). A systematic analysis of the 3'UTR of HNF4A mRNA reveals an interplay of regulatory elements including miRNA target sites. PLOS One, 6: e27438.

Liver specific transcriptional control
Kugler, W., Kaling, M., Roß, K., Wagner, U., Ryffel, G.U. (1990) BAP, a rat liver protein that activates transcription through a promoter element with similarity to the USF/MLTF binding site. Nucl. Acids Res. 18:6943-6951.

Kaling, M., Kugler, W., Roß, K., Zoidl, C., Ryffel, G.U. (1991) Liver-specific gene expression: A-activator-binding site, a promoter module present in vitellogenin and acute-phase genes. Mol. Cell. Biol. 11:93-101.

Drewes, T., Klein-Hitpass, L., Ryffel, G.U. (1991) Liver specific transcription factors of the HNF3-, C/EBP- and LFB1-families interact with the A-activator binding site. Nucl. Acids Res. 19:6383-6389.

Bartkowski, S., Zapp, D., Weber, H., Eberle, G., Zoidl, C., Senkel, S., Klein-Hitpass, L., Ryffel, G.U. (1993) Developmental regulation and tissue distribution of the liver transcription factor LFB1 (HNF1) in Xenopus laevis. Mol. Cell. Biol. 13:421-431.

Zapp, D., Bartkowski, S., Holewa, B., Zoidl, C., Klein-Hitpass, L., Ryffel, G.U. (1993) Elements and factors involved in tissue-specific and embryonic expression of the liver transcription factor LFB1 in Xenopus laevis. Mol. Cell. Biol. 13:6416-6426.

Transcriptional control by estrogen
Ryffel, G.U., Wahli, W., Weber, R. (1977) Quantitation of vitellogenin messenger RNA in the liver of male Xenopus toads during primary and secondary stimulation by estrogen. Cell 11:213-221.

Wahli, W., Dawid, I.B., Wyler, T., Jaggi, R.B., Weber, R., Ryffel, G.U. (1979) Vitellogenin in Xenopus laevis is encoded in a small family of genes. Cell 16:535-549.

Ryffel, G.U., Wyler, T., Muellener, D.B., Weber, R. (1980) Identification, organization and processing intermediates of the putative precursors of Xenopus vitellogenin messenger RNA. Cell 19:53-61.

Wahli, W., Dawid, I.B., Wyler, T., Weber, R., Ryffel, G.U. (1980) Comparative analysis of the structural organization of two closely related vitellogenin genes in Xenopus laevis. Cell 20:107-117.

Ryffel, G.U., Muellener, D.B., Wyler, T., Wahli, W., Weber, R. (1981) Transcription of single-copy vitellogenin gene of Xenopus involves expression of middle repetitive DNA. Nature 291:429-431.

Klein-Hitpaß, L., Schorpp, M., Wagner, U., Ryffel, G.U. (1986) An estrogen responsive element derived from the 5' flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cells. Cell 46:1053-1061.



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